Biosynthesis of l -Sorbose and l -Psicose Based on C—C Bond Formation Catalyzed by Aldolases in an Engineered Corynebacterium glutamicum Strain

Biosynthesis of l-Sorbose and l-Psicose Based on C-C Bond Formation Catalyzed by Aldolases in an Engineered Corynebacterium glutamicum Strain.

The property of loose stereochemical control at aldol products from aldolases helped to synthesize multiple polyhydroxylated compounds with nonnatural stereoconfiguration. In this study, we discovered for the first time that some fructose 1,6-diphosphate aldolases (FruA) and tagatose 1,6-diphosphate (TagA) aldolases lost their strict stereoselectivity when using l-glyceraldehyde and synthesized...

Enhanced production of L-lysine from Corynebacterium glutamicum by mutagenesis

Reducing lactate secretion by ldhA Deletion in L-glutamate- producing strain Corynebacterium glutamicum GDK-9

L-lactate is one of main byproducts excreted in to the fermentation medium. To improve L-glutamate production and reduce L-lactate accumulation, L-lactate dehydrogenase-encoding gene ldhA was knocked out from L-glutamate producing strain Corynebacterium glutamicum GDK-9, designated GDK-9ΔldhA. GDK-9ΔldhA produced approximately 10.1% more L-glutamate than the GDK-9, and yielded lower levels of s...

Phenotypic Assay on L - Sorbose

BIOCHEMISTRY. For the article ‘‘Kernel energy method: Application to insulin,’’ by Lulu Huang, Lou Massa, and Jerome Karle, which appeared in issue 36, September 6, 2005, of Proc. Natl. Acad. Sci. USA (102, 12690–12693; first published August 24, 2005; 10.1073 pnas.0506378102), the authors note an error in a funding acknowledgment: ‘‘L.M. thanks the National Institutes of Health for National In...

Tryptophan-Producing Corynebacterium glutamicum Strain

The aromatic amino acids are synthesized via a common biosynthetic pathway. A tryptophan-producing mutant of Corynebacterium glutamicum was genetically engineered to produce tyrosine or phenylalanine in abundance. To achieve this, three biosynthetic genes encoding the first enzyme in the common pathway, 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase (DS), and the branch-point enzymes chor...